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    ASTM E1498-92(2012)

    Standard Guide for Conducting Sexual Reproduction Tests with Seaweeds (Withdrawn 2021)

    Withdrawn Standard: ASTM E1498-92(2012) | Developed by Subcommittee: E50.47


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    Withdrawn Rationale:

    This guide covers procedures for obtaining laboratory data concerning the adverse effects of a test material added to dilution water on sexual reproduction by seaweeds. The exposure duration is species dependent and is followed by a period of development to allow the evidence of sexual reproduction to appear. There is no exposure to toxicants during the development period. This restricts the tests primarily to the events surrounding egg fertilization, and it minimizes any timelag effects on development that might interfere with correct enumeration of the number of sexual events that occurred. These procedures will probably be useful for conducting sexual reproduction toxicity tests with a variety of species of seaweeds, although modifications might be necessary.

    Formerly under the jurisdiction of Committee E50 on Environmental Assessment, Risk Management and Corrective Action, this guide was withdrawn in January 2021 in accordance with section 10.6.3 of the Regulations Governing ASTM Technical Committees, which requires that standards shall be updated by the end of the eighth year since the last approval date.

    1. Scope

    1.1 This guide covers procedures for obtaining laboratory data concerning the adverse effects of a test material added to dilution water on sexual reproduction by seaweeds. The exposure duration is species dependent and is followed by a period of development to allow the evidence of sexual reproduction to appear. There is no exposure to toxicants during the development period. This restricts the tests primarily to the events surrounding egg fertilization, and it minimizes any timelag effects on development that might interfere with correct enumeration of the number of sexual events that occurred. These procedures will probably be useful for conducting sexual reproduction toxicity tests with a variety of species of seaweeds, although modifications might be necessary.

    1.2 Other modifications of these procedures might be justified by special needs or circumstances. Although using appropriate procedures is more important than following prescribed procedures, the results of tests conducted using unusual procedures are not likely to be comparable to those of many other tests. Comparison of the results obtained using modified and unmodified versions of these procedures might provide useful information concerning new concepts and procedures for conducting sexual reproduction tests with seaweeds.

    1.3 These procedures are applicable to most chemicals, either individually or in formulations, commercial products, and known mixtures or whole effluents, as well as for use in testing surface waters. With appropriate modifications, these procedures can be used to study the effects of temperature, dissolved oxygen, pH, and such materials as leachates, oils, particulate matter, and sediments.

    1.4 The values stated in SI units are to be regarded as the standard.

    1.5 This guide is arranged as follows:

     

    Section

    Referenced Documents

     2  

    Terminology

     3  

    Summary of Guide

     4  

    Significance and Use

     5  

    Apparatus

     6  

     Facilities

     6.1

     Construction Materials

     6.2

     Test Chambers

     6.3

     Cleaning

     6.4

     Acceptability

     6.5

    Hazards

     7  

    Dilution and Culture Water

     8  

     Requirements

     8.1

     Source and Treatment

     8.2

    Test Material

     9  

     Single Chemicals

     9.1

     Stock Solutions

     9.2

     Effluents and Surface Waters

     9.3

     Test Concentrations

     9.4

    Test Organism

    10  

     Species

    10.1

     Life Stage

    10.2

     Source

    10.3

     Culture Nutrient Medium

    10.4

    Procedure

    11  

     Preparation of Plants for a Test

    11.1

     Test Conditions

    11.2

     Experimental Design

    11.3

    Acceptability of Test

    12  

    Calculation of Results

    13  

    Documentation

    14  

    Keywords

    15  

    Appendix

    X1 


    1.6 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. For specific hazards statements, see 6.4 and Section 7.



    2. Referenced Documents (purchase separately) The documents listed below are referenced within the subject standard but are not provided as part of the standard.

    ASTM Standards

    E380 Practice for Use of the International System of Units (SI) (the Modernized Metric System)

    E729 Guide for Conducting Acute Toxicity Tests on Test Materials with Fishes, Macroinvertebrates, and Amphibians

    E943 Terminology Relating to Biological Effects and Environmental Fate

    E1023 Guide for Assessing the Hazard of a Material to Aquatic Organisms and Their Uses

    E1192 Guide for Conducting Acute Toxicity Tests on Aqueous Ambient Samples and Effluents with Fishes, Macroinvertebrates, and Amphibians


    Referencing This Standard
    Link Here
    Link to Active (This link will always route to the current Active version of the standard.)

    DOI: 10.1520/E1498-92R12

    Citation Format

    ASTM E1498-92(2012), Standard Guide for Conducting Sexual Reproduction Tests with Seaweeds (Withdrawn 2021), ASTM International, West Conshohocken, PA, 2012, www.astm.org

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