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    ASTM E1440-91(2012)

    Standard Guide for Acute Toxicity Test with the Rotifer Brachionus (Withdrawn 2021)

    Withdrawn Standard: ASTM E1440-91(2012) | Developed by Subcommittee: E50.47


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    Withdrawn Rationale:

    This guide describes procedures for obtaining laboratory data concerning the acute toxicity of chemicals and aqueous effluents released into fresh, estuarine, or marine waters. Acute toxicity is measured by exposing Brachionus newly hatched from cysts to a series of toxicant concentrations under controlled conditions. This guide describes a test for using B. calyciflorus, a fresh water rotifer, and the Appendix describes modifications of this test for estuarine and marine waters using B. plicatilis. These procedures lead to an estimation of acute toxicity, including the concentration expected to kill 50 % of the test rotifers (LC50) in 24 h. Procedures not specifically stated in this guide should be conducted in accordance with Guide E729 and Guide E1192.

    Formerly under the jurisdiction of Committee E50 on Environmental Assessment, Risk Management and Corrective Action, this guide was withdrawn in January 2021 in accordance with section 10.6.3 of the Regulations Governing ASTM Technical Committees, which requires that standards shall be updated by the end of the eighth year since the last approval date.

    1. Scope

    1.1 This guide describes procedures for obtaining laboratory data concerning the acute toxicity of chemicals and aqueous effluents released into fresh, estuarine, or marine waters. Acute toxicity is measured by exposing Brachionus newly hatched from cysts to a series of toxicant concentrations under controlled conditions. This guide describes a test for using B. calyciflorus, a fresh water rotifer, and the Appendix describes modifications of this test for estuarine and marine waters using B. plicatilis. These procedures lead to an estimation of acute toxicity, including the concentration expected to kill 50 % of the test rotifers (LC50) in 24 h. Procedures not specifically stated in this guide should be conducted in accordance with Guide E729 and Guide E1192.

    1.2 Modifications of these procedures might be justified by special needs or circumstances. Although using appropriate procedures is more important than following prescribed procedures, the results of tests conducted using modified procedures might not be comparable to rotifer acute tests that follow the protocol described here. Comparison of the results using modified procedures might provide useful information concerning new concepts and procedures for conducting acute toxicity tests on chemicals and aqueous effluents.

    1.3 This guide is organized as follows:

    Section

     

    Scope

     1

    Referenced Documents

     2

    Terminology

     3

    Summary of Guide

     4

    Significance and Use

     5

    Apparatus

     6

    Dilution Water

     7

    Hazards

     8

    Test Material

     9

    Test Organisms

    10

    Test Procedure

    11

    Calculation of Results

    12

    Acceptability of the Test

    13

    Report

    14

    Keywords

    15


    1.4 These procedures are applicable to most chemicals, either individually or in formulations, commercial products, or mixtures. This guide can also be used to conduct investigations of the effects on rotifer survival of pH, hardness, and salinity and on materials such as aqueous effluents, leachates, oils, particulate matter, sediments, and surface waters. This guide might not be appropriate for materials with high oxygen demand, with high volatility, subject to rapid biological or chemical transformation, or that readily sorb to test chambers.

    1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. For specific hazards statements, see Section 8.



    2. Referenced Documents (purchase separately) The documents listed below are referenced within the subject standard but are not provided as part of the standard.

    ASTM Standards

    E380 Practice for Use of the International System of Units (SI) (the Modernized Metric System)

    E729 Guide for Conducting Acute Toxicity Tests on Test Materials with Fishes, Macroinvertebrates, and Amphibians

    E943 Terminology Relating to Biological Effects and Environmental Fate

    E1192 Guide for Conducting Acute Toxicity Tests on Aqueous Ambient Samples and Effluents with Fishes, Macroinvertebrates, and Amphibians


    Referencing This Standard
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    DOI: 10.1520/E1440-91R12

    Citation Format

    ASTM E1440-91(2012), Standard Guide for Acute Toxicity Test with the Rotifer Brachionus (Withdrawn 2021), ASTM International, West Conshohocken, PA, 2012, www.astm.org

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