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    ASTM E1415-91(2012)

    Standard Guide for Conducting Static Toxicity Tests With Lemna gibba G3 (Withdrawn 2021)

    Withdrawn Standard: ASTM E1415-91(2012) | Developed by Subcommittee: E50.47


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    Withdrawn Rationale:

    This guide describes procedures for obtaining laboratory data concerning the adverse effects of a text material added to growth medium on a certain species of duckweed (Lemna gibba G3) during a 7-day exposure using the static technique. These procedures will probably be useful for conducting toxicity tests with other species of duckweed and other floating vascular plants, although modifications might be necessary.

    Formerly under the jurisdiction of Committee E50 on Environmental Assessment, Risk Management and Corrective Action, this guide was withdrawn in January 2021 in accordance with section 10.6.3 of the Regulations Governing ASTM Technical Committees, which requires that standards shall be updated by the end of the eighth year since the last approval date.

    1. Scope

    1.1 This guide describes procedures for obtaining laboratory data concerning the adverse effects of a text material added to growth medium on a certain species of duckweed (Lemna gibba G3) during a 7-day exposure using the static technique. These procedures will probably be useful for conducting toxicity tests with other species of duckweed and other floating vascular plants, although modifications might be necessary.

    1.2 Special needs or circumstances might also justify modification of this standard. Although using appropriate procedures is more important than following prescribed procedures, results of tests conducted using unusual procedures are not likely to be comparable to results of many other tests. Comparison of results obtained using modified and unmodified versions of these procedures might provide useful information concerning new concepts and procedures for conducting tests with duckweed.

    1.3 The procedures in this guide are applicable to most chemicals, either individually or in formulations, commercial products, or known mixtures. With appropriate modifications these procedures can be used to conduct tests on temperature and pH and on such other materials as aqueous effluents (see also Guide E1192), leachates, oils, particulate matter, sediments and surface waters. These procedures do not specifically address effluents because to date there is little experience using duckweeds in effluent testing and such tests may pose problems with acclimation of the test organisms to the receiving water. Static tests might not be applicable to materials that have a high oxygen demand, are highly volatile, are rapidly biologically or chemically transformed in aqueous solution, or are removed from test solutions in substantial quantities by the test chambers or organisms during the test.

    1.4 Results of toxicity tests performed using the procedures in this guide should usually be reported in terms of the 7-day IC50 based on inhibition of growth. In some situations it might only be necessary to determine whether a specific concentration unacceptably affects the growth of the test species or whether the IC50 is above or below a specific concentration. Another end point that may be calculated is the no observed effect concentration (NOEC).

    1.5 The sections of this guide appear as follows:

      Title

    Section

     

    Referenced Documents

    2

    Terminology

    3

    Summary of Guide

    4

    Significance and Use

    5

    Hazards

    6

    Apparatus

    7

     Facilities

    7.1

     Test Chambers

    7.2

     Cleaning

    7.3

     Acceptability

    7.4

    Growth Medium

    8

     Test Material

    9

     General

    9.1

     Stock Solution

    9.2

     Test Concentration(s)

    9.3

    Test Organisms

    10

     Species

    10.1

     Source

    10.2

     Stock Culture

    10.3

    Procedure

    11

     Experimental Design

    11.1

     Temperature

    11.2

     Illumination

    11.3

     Beginning the Test

    11.4

     Duration of Test

    11.5

     Biological Data

    11.6

     Other Measurements

    11.7

    Analytical Methodology

    12

    Acceptability of Test

    13

    Calculation of Results

    14

    Report

    15


    1.6 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and to determine the applicability of regulatory limitations prior to use. Specific hazard statements are given in Section 6.



    2. Referenced Documents (purchase separately) The documents listed below are referenced within the subject standard but are not provided as part of the standard.

    ASTM Standards

    E729 Guide for Conducting Acute Toxicity Tests on Test Materials with Fishes, Macroinvertebrates, and Amphibians

    E943 Terminology Relating to Biological Effects and Environmental Fate

    E1023 Guide for Assessing the Hazard of a Material to Aquatic Organisms and Their Uses

    E1192 Guide for Conducting Acute Toxicity Tests on Aqueous Ambient Samples and Effluents with Fishes, Macroinvertebrates, and Amphibians

    E1218 Guide for Conducting Static Toxicity Tests with Microalgae


    Referencing This Standard
    Link Here
    Link to Active (This link will always route to the current Active version of the standard.)

    DOI: 10.1520/E1415-91R12

    Citation Format

    ASTM E1415-91(2012), Standard Guide for Conducting Static Toxicity Tests With Lemna gibba G3 (Withdrawn 2021), ASTM International, West Conshohocken, PA, 2012, www.astm.org

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