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This practice covered a typical procedure and guidelines for conducting the rat in vitro hepatocyte DNA repair assay. The procedures presented were based on similar protocols that were shown to be reliable.
Formerly under the jurisdiction of Committee F04 on Medical and Surgical Materials and Devices, this practice was withdrawn in December 2013. This standard was withdrawn with no replacement because the assay is based on very old work and detects a very small subset of genotoxin and carcinogens.
1.1 This practice covers a typical procedure and guidelines for conducting the rat in vitro hepatocyte DNA repair assay. The procedures presented here are based on similar protocols that have been shown to be reliable (1-6) .
1.2 Mention of trade names or commercial products are meant only as examples and not as endorsements. Other suppliers or manufacturers of equivalent products are acceptable.
1.3 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.
1.4 This standard does not purport to address all of the safety concerns associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.
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ASTM E1397-91(2008), Standard Practice for In Vitro Rat Hepatocyte DNA Repair Assay (Withdrawn 2014), ASTM International, West Conshohocken, PA, 2008, www.astm.orgBack to Top