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This practice covers the handling of microorganisms (bacteria, fungi, and protista), viruses, genetic elements (nucleic acids and plasmids), and animal and plant cell tissues (cell lines), during and after freezing and storage at cryogenic temperatures.
Formerly under the jurisdiction of Committee E48 on Biotechnology, this practice was withdrawn in January 2011 in accordance with section 10.5.3.1 of the Regulations Governing ASTM Technical Committees, which requires that standards shall be updated by the end of the eighth year since the last approval date.
1.1 This practice covers the handling of microorganisms (bacteria, fungi, and protista), viruses, genetic elements (nucleic acids and plasmids), and animal and plant cell tissues (cell lines), during and after freezing and storage at cryogenic temperatures.
1.2 This practice also covers the handling of microorganisms, viruses, and genetic elements in the host cell during and after freeze-drying.
1.3 While this practice does not cover the specific methodology used to freeze and freeze-dry microorganisms and cell lines, the safety aspects of handling microorganisms during freezing and freeze-drying procedures, and during storage at cryogenic temperatures, are covered. Other guidelines must also be adhered to regarding the handling of hazardous materials (2).
1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. For specific hazard statements see Section 6.
2. Referenced Documents (purchase separately) The documents listed below are referenced within the subject standard but are not provided as part of the standard.
E1564 Guide for Design and Maintenance of Low-Temperature Storage Facilities for Maintaining Cryopreserved Biological Materials
E1565 Guide for Inventory Control and Handling of Biological Materials Maintained at Low Temperatures
E1566 Guide for Handling Hazardous Biological Materials in Liquid Nitrogen
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ASTM E1342-97(2002), Standard Practice for Preservation by Freezing, Freeze-Drying, and Low Temperature Maintenance of Bacteria, Fungi, Protista, Viruses, Genetic Elements, and Animal and Plant Tissues (Withdrawn 2011), ASTM International, West Conshohocken, PA, 1997, www.astm.orgBack to Top