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    ASTM D932 - 20

    Standard Practice for Filamentous Iron Bacteria in Water and Water-Formed Deposits

    Active Standard ASTM D932 | Developed by Subcommittee: D19.24

    Book of Standards Volume: 11.02


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    Significance and Use

    5.1 Filamentous iron bacteria is a general classification for microorganisms that utilize ferrous iron as a source of energy and are characterized by the deposition of ferric hydroxide in their mucilaginous sheaths. The process is continuous with these growths, and over a period of time large accumulations of slimy brown deposits can occur. Iron bacteria may clog water lines, reduce heat transfer, and cause staining; objectionable odors may arise following death of the bacteria. The organic matter in the water is consequently increased, and this in turn favors the multiplication of other bacteria.

    1. Scope

    1.1 This practice covers the determination of filamentous iron bacteria (FIB) by microscopic examination. This practice provides for the identification of the following genera of bacteria found in water and water-formed deposits: Siderocapsa, Gallionella (Dioymohelix), Sphaerotilus, Crenothrix, Leptothrix, and Clonothrix.

    1.2 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.

    1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.

    1.4 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.


    2. Referenced Documents (purchase separately) The documents listed below are referenced within the subject standard but are not provided as part of the standard.

    ASTM Standards

    D887 Practices for Sampling Water-Formed Deposits

    D1129 Terminology Relating to Water

    D1193 Specification for Reagent Water

    D3370 Practices for Sampling Water from Closed Conduits

    D5465 Practice for Determining Microbial Colony Counts from Waters Analyzed by Plating Methods


    ICS Code

    ICS Number Code 13.060.50 (Examination of water for chemical substances)

    UNSPSC Code

    UNSPSC Code


    Referencing This Standard
    Link Here
    Link to Active (This link will always route to the current Active version of the standard.)

    DOI: 10.1520/D0932-20

    Citation Format

    ASTM D932-20, Standard Practice for Filamentous Iron Bacteria in Water and Water-Formed Deposits, ASTM International, West Conshohocken, PA, 2020, www.astm.org

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