ASTM D8238 - 18

    Standard Test Method for Immunological Assay to Quantify Extractable Guayule Natural Rubber (GNR) Proteins

    Active Standard ASTM D8238 | Developed by Subcommittee: D11.40

    Book of Standards Volume: 09.02


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    Significance and Use

    5.1 NRL derived from Hevea contains over 200 proteins a number of which are allergens capable of eliciting Type 1 anaphylactic reactions. Guayule Natural Rubber derived from the Parthenium argentatum plant contains many fewer proteins and at much lower levels than Hevea NRL. While there have been no reports of allergic reactions to GNR proteins at present, they have been shown to induce antibody production in exposed individuals.4 An immunological assay that tests for the presence of GNR has the advantage of providing the specificity and sensitivity that other ASTM standards such as Test Methods D5712 and D6499 do not offer.

    5.2 This test method describes an immunological method for the quantitation of Guayule natural rubber proteins using rabbit anti-GNR serum. Rabbits immunized with GNR proteins react to the majority of the proteins present, and their sera have the capability to detect most if not all of the proteins in GNR. This test method may be used to determine if a test item contains material derived from a natural source.

    1. Scope

    1.1 This test method covers an immunological method to determine the amount of protein in Guayule Natural Rubber (GNR) and its products using rabbit antisera specific for Guayule Natural Rubber latex proteins. This immunoassay procedure quantitatively measures the level of GNR proteins in solution using an inhibition format. The samples may include but are not restricted to, gloves or other GNR product extracts, and liquid GNR samples which have been collected in order to measure the protein levels.

    1.2 For the purpose of this test method, the range of protein will be measured in terms of microgram to milligram quantities.

    1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.

    1.4 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.


    2. Referenced Documents (purchase separately) The documents listed below are referenced within the subject standard but are not provided as part of the standard.

    ASTM Standards

    D5712 Test Method for Analysis of Aqueous Extractable Protein in Latex, Natural Rubber, and Elastomeric Products Using the Modified Lowry Method

    D6499 Test Method for Immunological Measurement of Antigenic Protein in Hevea Natural Rubber (HNR) and its Products

    D7427 Test Method for Immunological Measurement of Four Principal Allergenic Proteins (Hev b 1, 3, 5 and 6.02) in Hevea Natural Rubber and Its Products Derived from Latex

    E177 Practice for Use of the Terms Precision and Bias in ASTM Test Methods

    E691 Practice for Conducting an Interlaboratory Study to Determine the Precision of a Test Method


    ICS Code

    ICS Number Code 83.040.01 (Raw materials for rubber and plastics in general)

    Referencing This Standard
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    DOI: 10.1520/D8238-18

    Citation Format

    ASTM D8238-18, Standard Test Method for Immunological Assay to Quantify Extractable Guayule Natural Rubber (GNR) Proteins, ASTM International, West Conshohocken, PA, 2018, www.astm.org

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