ASTM D8001 - 16e1

    Standard Test Method for Determination of Total Nitrogen, Total Kjeldahl Nitrogen by Calculation, and Total Phosphorus in Water, Wastewater by Ion Chromatography

    Active Standard ASTM D8001 | Developed by Subcommittee: D19.06

    Book of Standards Volume: 11.02

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    Significance and Use

    5.1 This test method allows the simultaneous determination of total nitrogen and total phosphorous from one sample digestion step.

    5.2 This test method measures oxidized ammonia and organic nitrogen (as nitrate) and soluble nitrate simultaneously. By subtracting the nitrate + nitrite value from a non-digested sample gives a TKN:

    Equation D8001-16E01_4

    Equation D8001-16E01_5

    When using this test method:

    Equation D8001-16E01_6

    Equation D8001-16E01_7


    TN   =   total nitrogen, and
    TKN   =   total Kjeldahl nitrogen.

    1. Scope

    1.1 This test method is applicable for the analysis total nitrogen (organic nitrogen + ammonia-N + nitrate-N + nitrite-N) as nitrate and total phosphorus as orthophosphate in unfiltered water samples by alkaline persulfate digestion followed by ion chromatography (IC).

    1.2 Total Kjeldahl nitrogen (TKN) is determined by the calculation. To determine TKN subtract the nitrate-N and nitrite-N in a digested sample from a non-digested sample (see Section 4, Summary of Test Method).

    1.3 The limit of detection (LOD), limit of quantitation (LOQ), and reporting range in Table 1 are based on the two-step process for this test method: digestion and analytical step. Because the digestion step requires a sample dilution, the LOD and LOQ are higher than undigested samples. The reporting range, LOD, and LOQ can be modified and perhaps improved depending on several factors (see Section 6, Interferences).

    (A) Ten calibration levels, each injected in duplicate.
    (B) LOD calculated as 3 × S/N.
    (C) LOQ calculated as 10 × S/N.

    Equation D8001-16E01_1


    A   =   the average method blank concentration,
    Equation D8001-16E01_2   =   the student’s t-value for the single-tailed 99th percentile t statistic a standard deviation estimate with n – 1 degrees of freedom, and
    Sb   =   the sample standard deviation of the replicate blank analyses.
    (E) Phosphate LOD/LOQ was calculated based on a dilution factor of 15× relative to the system concentrations.

    1.4 The method detection limits (MDL) are shown for reference. The digestion reagent contains background nitrate and results in higher detection limits. MDL will be shown after the interlaboratory study (ILS) is completed.

    1.5 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.

    1.6 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

    2. Referenced Documents (purchase separately) The documents listed below are referenced within the subject standard but are not provided as part of the standard.

    ASTM Standards

    D1129 Terminology Relating to Water

    D1193 Specification for Reagent Water

    D2777 Practice for Determination of Precision and Bias of Applicable Test Methods of Committee D19 on Water

    D3856 Guide for Management Systems in Laboratories Engaged in Analysis of Water

    D4327 Test Method for Anions in Water by Suppressed Ion Chromatography

    D5810 Guide for Spiking into Aqueous Samples

    D5847 Practice for Writing Quality Control Specifications for Standard Test Methods for Water Analysis

    D6299 Practice for Applying Statistical Quality Assurance and Control Charting Techniques to Evaluate Analytical Measurement System Performance

    D6792 Practice for Quality System in Petroleum Products and Lubricants Testing Laboratories

    ICS Code

    ICS Number Code 13.060.50 (Examination of water for chemical substances)

    UNSPSC Code

    UNSPSC Code

    Referencing This Standard
    Link Here
    Link to Active (This link will always route to the current Active version of the standard.)

    DOI: 10.1520/D8001-16E01

    Citation Format

    ASTM D8001-16e1, Standard Test Method for Determination of Total Nitrogen, Total Kjeldahl Nitrogen by Calculation, and Total Phosphorus in Water, Wastewater by Ion Chromatography, ASTM International, West Conshohocken, PA, 2016,

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