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Formerly under the jurisdiction of Committee D19 on Water, this Test Method was withdrawn in 2011 in accordance with section 10.5.3.1 of the Regulations Governing ASTM Technical Committees, which requires that standards shall be updated by the end of the eighth year since the last approval date.
1.1 This test method can enumerate Clostridium perfringens spores and vegetative cells from marine water, sediment, wastewater, ambient water, and drinking water. Since C. perfringens spores are present in large numbers in human and animal wastes and are resistant to wastewater treatment practices, extremes in temperature, and environmental stress, they are an indicator of present fecal contamination as well as a conservative tracer of past fecal contamination. It is the user's responsibility to ensure the validity of this test method for waters of untested matrices.
1.2 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.
2. Referenced Documents (purchase separately) The documents listed below are referenced within the subject standard but are not provided as part of the standard.
D1129 Terminology Relating to Water
D1193 Specification for Reagent Water
D1888 Methods of Test for Particulate and Dissolved Matter in Water
D2777 Practice for Determination of Precision and Bias of Applicable Methods of Committee D19 on Water
D3370 Practices for Sampling Water from Closed Conduits
D3863 Test Method for Retention Characteristics of 0.4 to 0.45-m Membrane Filters Used in Routine Filtration Procedures for the Evaluation of Microbiological Water Quality
D3870 Practice for Establishing Performance Characteristics for Colony Counting Method in Bacteriology
D5465 Practice for Determining Microbial Counts from Waters Analyzed by Plating Methods
E200 Practice for Preparation, Standardization, and Storage of Standard and Reagent Solutions for Chemical Analysis
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ASTM D5916-96(2002), Standard Test Method for Detection and Enumeration of Clostridium perfringens from Water and Extracted Sediments by Membrane Filtration (MF) (Withdrawn 2011), ASTM International, West Conshohocken, PA, 1996, www.astm.orgBack to Top