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Significance and Use
4.1 Numerous ASTM test methods and practices (for example: Test Methods and , and Practices and ) report colony counts as their measured parameter.
4.2 These practices provide a uniform set of counting, calculating, and reporting procedures for ASTM test methods in microbiology.
A—Counting Colonies on Membrane Filters
B—Counting Colonies on Pour Plates
C—Counting Colonies on Spread Plates
4.3 The counting rules provide a best attainable estimate of microorganisms in the sample, since the samples cannot be held and reanalyzed at a later date.
1.1 These practices cover recommended procedures for counting colonies and reporting colony-forming units (CFU) on membrane filters (MF) and standard pour and spread plates.
1.2 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.
1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.
1.4 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.
2. Referenced Documents (purchase separately) The documents listed below are referenced within the subject standard but are not provided as part of the standard.
D1129 Terminology Relating to Water
D5259 Test Method for Isolation and Enumeration of Enterococci from Water by the Membrane Filter Procedure
D5392 Test Method for Isolation and Enumeration of Escherichia coli in Water by the Two-Step Membrane Filter Procedure
D6161 Terminology Used for Microfiltration, Ultrafiltration, Nanofiltration, and Reverse Osmosis Membrane Processes
D6974 Practice for Enumeration of Viable Bacteria and Fungi in Liquid FuelsFiltration and Culture Procedures
E2563 Practice for Enumeration of Non-Tuberculosis Mycobacteria in Aqueous Metalworking Fluids by Plate Count Method
Other StandardsAPHA 9215
ICS Number Code 07.100.20 (Microbiology of water)
|Link to Active (This link will always route to the current Active version of the standard.)|
ASTM D5465-16(2020), Standard Practices for Determining Microbial Colony Counts from Waters Analyzed by Plating Methods, ASTM International, West Conshohocken, PA, 2020, www.astm.orgBack to Top