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    ASTM D5412 - 93(2017)e1

    Standard Test Method for Quantification of Complex Polycyclic Aromatic Hydrocarbon Mixtures or Petroleum Oils in Water

    Active Standard ASTM D5412 | Developed by Subcommittee: D19.06

    Book of Standards Volume: 11.02

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    Significance and Use

    5.1 This test method is useful for characterization and rapid quantification of PAH mixtures including petroleum oils, fuels, creosotes, and industrial organic mixtures, either waterborne or obtained from tanks.

    5.2 The unknown PAH mixture is first characterized by its fluorescence emission and synchronous scanning spectra. Then a suitable site-specific calibration standard with similar spectral characteristics is selected as described in Annex A1. This calibration standard may also be well-characterized by other independent methods such as gas chromatography (GC), GC-mass spectrometry (GC-MS), or high performance liquid chromatography (HPLC). Some suggested independent analytical methods are included in References (1-7)4 and Test Method D4657. Other analytical methods can be substituted by an experienced analyst depending on the intended data quality objectives. Peak maxima intensities of appropriate fluorescence emission spectra are then used to set up suitable calibration curves as a function of concentration. Further discussion of fluorescence techniques as applied to the characterization and quantification of PAHs and petroleum oils can be found in References (8-18).

    5.3 For the purpose of the present test method polynuclear aromatic hydrocarbons are defined to include substituted polycyclic aromatic hydrocarbons with functional groups such as carboxyl acid, hydroxy, carbonyl and amino groups, and heterocycles giving similar fluorescence responses to PAHs of similar molecular weight ranges. If PAHs in the more classic definition, that is, unsubstituted PAHs, are desired, chemical reactions, extractions, or chromatographic procedures may be required to eliminate these other components. Fortunately, for the most commonly expected PAH mixtures, such substituted PAHs and heterocycles are not major components of the mixtures and do not cause serious errors.

    1. Scope

    1.1 This test method covers a means for quantifying or characterizing total polycyclic aromatic hydrocarbons (PAHs) by fluorescence spectroscopy (Fl) for waterborne samples. The characterization step is for the purpose of finding an appropriate calibration standard with similar emission and synchronous fluorescence spectra.

    1.2 This test method is applicable to PAHs resulting from petroleum oils, fuel oils, creosotes, or industrial organic mixtures. Samples can be weathered or unweathered, but either the same material or appropriately characterized site-specific PAH or petroleum oil calibration standards with similar fluorescence spectra should be chosen. The degree of spectral similarity needed will depend on the desired level of quantification and on the required data quality objectives.

    1.3 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.

    1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.

    1.5 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

    2. Referenced Documents (purchase separately) The documents listed below are referenced within the subject standard but are not provided as part of the standard.

    ASTM Standards

    D1129 Terminology Relating to Water

    D1193 Specification for Reagent Water

    D2777 Practice for Determination of Precision and Bias of Applicable Test Methods of Committee D19 on Water

    D3325 Practice for Preservation of Waterborne Oil Samples

    D3326 Practice for Preparation of Samples for Identification of Waterborne Oils

    D3415 Practice for Identification of Waterborne Oils

    D3650 Test Method for Comparison of Waterborne Petroleum Oils By Fluorescence Analysis

    D4489 Practices for Sampling of Waterborne Oils

    D4657 Test Method for Polynuclear Aromatic Hydrocarbons in Water

    E131 Terminology Relating to Molecular Spectroscopy

    E169 Practices for General Techniques of Ultraviolet-Visible Quantitative Analysis

    E275 Practice for Describing and Measuring Performance of Ultraviolet and Visible Spectrophotometers

    E388 Test Method for Wavelength Accuracy and Spectral Bandwidth of Fluorescence Spectrometers

    E578 Test Method for Linearity of Fluorescence Measuring Systems

    E579 Test Method for Limit of Detection of Fluorescence of Quinine Sulfate in Solution

    ICS Code

    ICS Number Code 13.060.50 (Examination of water for chemical substances)

    UNSPSC Code

    UNSPSC Code 15101508(Crude oil)

    Referencing This Standard
    Link Here
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    DOI: 10.1520/D5412-93R17E01

    Citation Format

    ASTM D5412-93(2017)e1, Standard Test Method for Quantification of Complex Polycyclic Aromatic Hydrocarbon Mixtures or Petroleum Oils in Water, ASTM International, West Conshohocken, PA, 2011, www.astm.org

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