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    ASTM D5246 - 19

    Standard Test Method for Isolation and Enumeration of Pseudomonas aeruginosa from Water

    Active Standard ASTM D5246 | Developed by Subcommittee: D19.24

    Book of Standards Volume: 11.02

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    Significance and Use

    5.1 P. aeruginosa is an opportunistic pathogen and has been linked as the causative agent of numerous infections that may be transmitted through a contaminated water supply to a susceptible host.

    Note 1: Fecal waste is >95 % E. coli which is found in humans and warm bloodied animals.

    5.2 The membrane filtration procedure described is a rapid and reliable test method of detecting P. aeruginosa in water.

    1. Scope

    1.1 The test method covers the isolation and enumeration of Pseudomonas aeruginosa. Testing was performed on spiked samples using reagent grade water as the diluent from surface waters; recreational waters; ground water, water supplies; especially rural nonchlorinated sources; waste water; and saline waters. The detection limit of this test method is one microorganism per 100 mL.

    1.2 This test method was used successfully with reagent water. It is the user's responsibility to ensure the validity of this test method for surface waters, recreational waters, ground water, rural nonchlorinated sources; waste water; and saline waters.

    1.3 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.

    1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. Specific hazard statements are given in Section 10.

    1.5 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

    2. Referenced Documents (purchase separately) The documents listed below are referenced within the subject standard but are not provided as part of the standard.

    ASTM Standards

    D1129 Terminology Relating to Water

    D1193 Specification for Reagent Water

    D2777 Practice for Determination of Precision and Bias of Applicable Test Methods of Committee D19 on Water

    D3370 Practices for Sampling Water from Flowing Process Streams

    ICS Code

    ICS Number Code 07.100.20 (Microbiology of water)

    Referencing This Standard
    Link Here
    Link to Active (This link will always route to the current Active version of the standard.)

    DOI: 10.1520/D5246-19

    Citation Format

    ASTM D5246-19, Standard Test Method for Isolation and Enumeration of Pseudomonas aeruginosa from Water, ASTM International, West Conshohocken, PA, 2019, www.astm.org

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