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This test method describes a procedure for detection and enumeration of aquatic bacteria by the use of an acridine-orange epifluorescence direct-microscopic counting procedure.
Formerly under the jurisdiction of Committee D19 on Water, this test method was withdrawn in April 2019. This standard was withdrawn without replacement due to its limited use by the industry.
1.1 This test method describes a procedure for detection and enumeration of aquatic bacteria by the use of an acridine-orange epifluorescence direct-microscopic counting procedure. It is applicable to environmental waters.
1.2 Certain types of debris and other microorganisms may fluoresce in acridine orange-stained smears.
1.3 The test method requires a trained microbiologist or technician who is capable of distinguishing bacteria from other fluorescing bodies on the basis of morphology when viewed at higher magnifications.2
1.4 Use of bright light permits differentiation of single bacteria where reduced formazan is deposited at the polar ends.
1.5 Approximately 104 cells/mL are required for detection by this test method.2
1.6 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.
1.7 This standard does not purport to address the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.
2. Referenced Documents (purchase separately) The documents listed below are referenced within the subject standard but are not provided as part of the standard.
D1129 Terminology Relating to Water
D1193 Specification for Reagent Water
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ASTM D4455-85(2014), Standard Test Method for Enumeration of Aquatic Bacteria by Epifluorescence Microscopy Counting Procedure (Withdrawn 2019), ASTM International, West Conshohocken, PA, 2014, www.astm.orgBack to Top