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    ASTM D4198 - 20

    Standard Test Methods for Evaluating Absorbent Pads Used with Membrane Filters for Bacteriological Analysis and Growth 

    Active Standard ASTM D4198 | Developed by Subcommittee: D19.08

    Book of Standards Volume: 11.02


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    Significance and Use

    5.1 These test methods are appropriate for qualifying absorbent pads used with membrane filters for bacteriological enumeration.

    5.1.1 The test methods described are applicable to quality control testing of absorbent pads by the suppliers and users of these pads and to specification testing of absorbent pads intended for use with membrane filters in bacteriological enumeration.

    5.2 Other pure culture organisms and their appropriate culture medium may be substituted for the E. coli and M-FC media for specification testing, as required.

    1. Scope

    1.1 These test methods cover the determination of the nutrient-holding capacity and the toxic or nutritive effect on bacterial growth of organisms retained on a membrane filter, when the absorbent pad being tested is used as a nutrient reservoir and medium supply source for the retained bacteria.

    1.2 The tests described are conducted on 47 mm diameter disks, although other size disks may be employed for bacterial culture techniques.

    1.3 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.

    1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.

    1.5 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.


    2. Referenced Documents (purchase separately) The documents listed below are referenced within the subject standard but are not provided as part of the standard.

    ASTM Standards

    D1129 Terminology Relating to Water

    D1193 Specification for Reagent Water

    D3508 Method for Evaluating Water Testing Membrane Filters for Fecal Coliform Recovery


    UNSPSC Code

    UNSPSC Code 41104921(Laboratory membrane filters)


    Referencing This Standard
    Link Here
    Link to Active (This link will always route to the current Active version of the standard.)

    DOI: 10.1520/D4198-20

    Citation Format

    ASTM D4198-20, Standard Test Methods for Evaluating Absorbent Pads Used with Membrane Filters for Bacteriological Analysis and Growth , ASTM International, West Conshohocken, PA, 2020, www.astm.org

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