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This practice covers determination of the quantitative and qualitative species composition of fish in a specified area. The successful use of this technique is dependent on: (1) preventing fish from escaping the sample area and (2) retrieving all affected fish, which may take up to three days. This practice is useful in both short- and long-term studies for management and impact assessment purposes. The sample area is blocked off with a small mesh net(s) and the volume of water to be treated is calculated. The required quantity of rotenone is diluted and distributed throughout the water column in the sample area. All fish should be affected and they should be collected for processing.
This abstract is a brief summary of the referenced standard. It is informational only and not an official part of the standard; the full text of the standard itself must be referred to for its use and application. ASTM does not give any warranty express or implied or make any representation that the contents of this abstract are accurate, complete or up to date.
1.1 This practice covers determination of the quantitative and qualitative species composition of fish in a specified area. The successful use of this technique is dependent on: (1) preventing fish from escaping the sample area and (2) retrieving all affected fish, which may take up to three days.
1.2.1 Easily detoxified.
1.2.2 All native freshwater fish are susceptible, but it has low toxicity to mammals and birds.
1.2.3 At low concentrations fish toxicity depends on species, age, and size.
1.2.4 The suffocating action is reversible.
1.3.1 It is less effective in cold (below 20°C) and highly alkaline water.
1.3.2 Smaller fish and those without air bladders usually do not float.
1.3.3 Completely random selection of sample areas is not possible.
1.3.4 Overkill beyond sample area can sometimes occur.
1.3.5 Food web organisms may be eliminated.
1.4 Applications—This practice is useful in both short- and long-term studies for management and impact assessment purposes. It is adaptable to both lotic and lentic situations in littoral and limnetic areas.
1.5 The values stated in inch-pound units are to be regarded as standard. The values given in parentheses are mathematical conversions to SI units that are provided for information only and are not considered standard.
1.6 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. For specific hazards, see Section .
1.7 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.
2. Referenced Documents (purchase separately) The documents listed below are referenced within the subject standard but are not provided as part of the standard.
D1129 Terminology Relating to Water
D1193 Specification for Reagent Water
ICS Number Code 67.120.30 (Fish and fishery products)
UNSPSC Code 50121500(Fish); 77101500(Environmental impact assessment)
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ASTM D4131-19, Standard Practice for Sampling Fish with Rotenone, ASTM International, West Conshohocken, PA, 2019, www.astm.orgBack to Top