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    ASTM D3978 - 21

    Standard Practice for Algal Growth Potential Testing with Pseudokirchneriella subcapitata

    Active Standard ASTM D3978 | Developed by Subcommittee: E50.47

    Book of Standards Volume: 11.06

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    Significance and Use

    4.1 The significance of measuring algal growth potential in water samples is that differentiation can be made between the nutrients of a sample determined by chemical analysis and the nutrients that are actually available for algal growth. The addition of nutrients (usually nitrogen and phosphorus singly or in combination) to the sample can give an indication of which nutrient(s) is (are) limiting for algal growth (1,10,11,12,13,14).

    1. Scope

    1.1 This practice measures, by Pseudokirchnereilla subcapitata growth response, the biological availability of nutrients, as contrasted with chemical analysis of the components of the sample. This practice is useful for assessing the impact of nutrients, and changes in their loading, upon freshwater algal productivity. Other laboratory or indigenous algae can be used with this practice. However, Pseudokirchnereilla subcapitata must be cultured as a reference alga along with the alternative algal species.

    1.2 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. For a specific precautionary statement, see Section 15.

    1.3 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

    2. Referenced Documents (purchase separately) The documents listed below are referenced within the subject standard but are not provided as part of the standard.

    ASTM Standards

    D1193 Specification for Reagent Water

    E1023 Guide for Assessing the Hazard of a Material to Aquatic Organisms and Their Uses

    E1733 Guide for Use of Lighting in Laboratory Testing

    ICS Code

    ICS Number Code 13.060.70 (Examination of water for biological properties)

    UNSPSC Code

    UNSPSC Code 77101505(Environmental monitoring)

    Referencing This Standard
    Link Here
    Link to Active (This link will always route to the current Active version of the standard.)

    DOI: 10.1520/D3978-21

    Citation Format

    ASTM D3978-21, Standard Practice for Algal Growth Potential Testing with Pseudokirchneriella subcapitata, ASTM International, West Conshohocken, PA, 2021, www.astm.org

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