If you are an ASTM Compass Subscriber and this document is part of your subscription, you can access it for free at ASTM Compass
    ASTM D3731 - 20

    Standard Practices for Measurement of Chlorophyll Content of Algae in Surface Waters

    Active Standard ASTM D3731 | Developed by Subcommittee: D19.24

    Book of Standards Volume: 11.02


      Format Pages Price  
    PDF 5 $52.00   ADD TO CART
    Hardcopy (shipping and handling) 5 $52.00   ADD TO CART
    Standard + Redline PDF Bundle 10 $62.00   ADD TO CART



    Significance and Use

    5.1 Data on the chlorophyll content of the algae have the following applications:

    5.1.1 To provide estimates of algal biomass and productivity.

    5.1.2 To provide general information on the taxonomic composition (major groups) of the algae, based on the relative amounts of chlorophyll a, b, and c, and the physiological condition of algal communities, which is related to the relative abundance of pheopigments.

    5.1.3 To determine long-term trends in water quality.

    5.1.4 To determine the trophic status of surface waters.

    5.1.5 To detect adverse effects of pollutants on plankton and periphyton in receiving waters.

    5.1.6 To determine maximum growth rates and yields in algal growth potential tests.

    1. Scope

    1.1 These practices include the extraction and the measurement of chlorophyll a, b, and c, and pheophytin a in freshwater and marine plankton and periphyton. Three practices are provided as follows:

    1.1.1 Spectrophotometric, trichromatic practice for measuring chlorophyll a, b, and c.

    1.1.2 Spectrophotometric, monochromatic practice for measuring chlorophyll a corrected for pheophytin a; and for measuring pheophytin a.

    1.1.3 Fluorometric practice for measuring chlorophyll a corrected for pheophytin a; and for measuring pheophytin a.

    1.2 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.

    1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. Specific precautionary statements are given in Section 8.

    1.4 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.


    2. Referenced Documents (purchase separately) The documents listed below are referenced within the subject standard but are not provided as part of the standard.

    ASTM Standards

    D1129 Terminology Relating to Water

    D1193 Specification for Reagent Water


    ICS Code

    ICS Number Code 13.060.10 (Water of natural resources)

    UNSPSC Code

    UNSPSC Code 77121701(Surface water pollution monitoring or control services)


    Referencing This Standard
    Link Here
    Link to Active (This link will always route to the current Active version of the standard.)

    DOI: 10.1520/D3731-20

    Citation Format

    ASTM D3731-20, Standard Practices for Measurement of Chlorophyll Content of Algae in Surface Waters, ASTM International, West Conshohocken, PA, 2020, www.astm.org

    Back to Top