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An intermittent-flow, proportional diluter system has been designed for the study of synergistic and antagonistic effects of two toxicants on aquatic organisms. The system consists of two diluters mounted side by side, each of which produces three toxicant concentrations plus a solvent control. Below the diluters are two additional mixing, sample-splitting levels. The first divides each concentration of each toxicant into four equal volumes. The second consists of 16 compartments, each of which receives a measured volume from 1 of the 4 concentrations of each toxicant. This produces a 4 by 4 matrix of all 16 possible combinations. This final level also serves as a series of sample-replicating chambers from which the toxicant mixtures flow to 32 test aquaria.
water analysis, toxicology, dilution, bioassay, toxicity, tests, interactions, flow-through, diluter, synergism
Research assistant, University of Georgia, Athens, Ga.