SYMPOSIA PAPER Published: 01 January 1990
STP20116S

Reduction in the Numbers of Antibody-Producing Cells in Rainbow Trout, , Exposed to Sublethal Doses of Phenol Before Bath Immunization

Source

The numbers of splenic antibody-producing cells were reduced in groups of fish given a 2, 5, or 30 min bath exposure to 10 ppm phenol before immunization by bath with the bacterin, Yersinia ruckeri O-antigen. The lethal time of 50% (LT50) for the 10 ppm phenol bath was 45 min. The passive hemolytic plaque assay was used to monitor the numbers of antibody-producing cells in the phenol-pretreated and sham-treated fish 14 or 21 days after contaminant exposure and 5 min bath immunizations at 10 or 100 μg/mL. These results, documenting a suppression of the immune response to bath immunization following a brief exposure to a toxicant, may help to explain delayed mortalities where pollution is implicated.

Author Information

Anderson, DP
U.S. Fish and Wildlife Service, National Fish Health Research Laboratory, Kearneysville, WV
Dixon, OW
U.S. Fish and Wildlife Service, National Fish Health Research Laboratory, Kearneysville, WV
van Muiswinkel, WB
Agricultural University, Zodiac, Wageningen, The Netherlands
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Details
Developed by Committee: E47
Pages: 331–337
DOI: 10.1520/STP20116S
ISBN-EB: 978-0-8031-5151-2
ISBN-13: 978-0-8031-1460-9