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    Resolution of DL-Amino Acids by Capillary Zone Electrophoresis Using Chiral Electrolytes

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    By combining the separation power of high-voltage capillary zone elctrophoresis with the detection sensitivity of laser-induced fluorescence, subfemtomole amounts of racemic mixtures of labelled amino acids can be completely resolved in short periods of time. The separation is based on the diastereomeric interaction between the amino acids and a chiral Cu(II)-aspartame complex present in the support electrolyte. Effects of electrolyte composition, pH, and temperature are described and discussed as well as linearity and sensitivity of response.


    electrokinetic separation, laser induced fluorescence detection, chirality, amino acid, aspartame, resolution, sensitivity, temperature, micelle

    Author Information:

    Gozel, P

    Zare, RN
    Stanford University, Stanford, CA

    Committee/Subcommittee: E13.06

    DOI: 10.1520/STP19689S