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Various components of a conventional fluorometer are discussed. Several approaches to optimization of some of these components are discussed by citing specific research examples. The four areas of research that are described include: stabilization of a xenon arc lamp, improvements in sample chemistry, increased detector selectivity, and development of data reduction strategies for complex data. Improvements in the fluorescence measurement process are cited with each example.
fluorescence, multidimensional fluorescence, fluorescence-detected circular dichroism, sample deoxygenation, cyclodextrin complexes, polynuclear aromatic hydrocarbons, data reduction, chemometrics
Emory University, Atlanta, GA