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Suspension cultures of Rosa cultivar (cv.) Paul's Scarlet were used as a model system to examine the metabolism of 1,3-dinitrobenzene (DNB), an industrial waste compound. In a 3-day period, 90% of the DNB supplied (96 nmol) was metabolized by approximately 12 g (fresh weight) of cells. The primary end product of DNB metabolism partitioned into the insoluble residue fraction of the extracted cells. This study demonstrates the usefulness of plant tissue cultures in evaluating the chemical influence of plants on xenobiotics.
suspension cultures, dinitrobenzene, metabolism, plant tissue culture
Biologist, U.S. Environmental Protection Agency, Environmental Research Laboratory, Corvallis, OR
Professor of botany, University of Oklahoma, Norman, OK