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    Ecotoxicological Assessment of Toluene and Cadmium Using Plant Cell Cultures

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    Cell suspension cultures from a variety of plant species can be grown from callus for use in ecotoxicological testing. The effects of toluene and cadmium on plant cell growth were investigated. The accumulation of cadmium in the soluble cell “cytoplasmic” and in the cell residue compartment in two cell types was also verified by elemental analysis using inductively coupled atomic plasma emission spectroscopy (ICPAES). Plant cell cultures in suspension can be most useful in tracing the fate of labeled toxic chemicals and in an isolated environment. Both tracer fate tests and toxicity tests with plant cell cultures can then aid in the optimal design of more costly experiments with whole plants. Callus and suspension cultures can be maintained with their individual characteristics through many cell transfers. Alternately, these characteristics can be preserved by placing appropriately treated cells at liquid nitrogen temperatures. A majority of these cultures can be grown in standard media that can be made in individual labs or purchased from regular suppliers. The use of standard cultures make it possible to address comparability for routine ecotoxicological testing of chemicals between different labs.


    plant cell suspension cultures, toxicity testing, metabolism, toluene, cadmium

    Author Information:

    Reporter, M
    NSI Technological Services, USEPA-ERL, Corvallis, OR

    Robideaux, M
    NSI Technological Services, USEPA-ERL, Corvallis, OR

    Wickster, P
    NSI Technological Services, USEPA-ERL, Corvallis, OR

    Wagner, J
    Ames Laboratory IAG, USEPA-ERL,

    Kapustka, L
    U.S. EPA, Environmental Research Laboratory, Corvallis, OR

    Committee/Subcommittee: E47.09

    DOI: 10.1520/STP19518S