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    The Application of Avoidance/Preference Testing in Aquatic Toxicology

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    Preference/avoidance behavioral testing was evaluated as a method to determine the effects of low levels of olfactory attractant, single toxicant, and mixed effluent wastes from domestic and industrial dischargers on selected organisms. Various dilutions of effluents and a reference toxicant (phenol) were tested using linear velocity, locational position, and other factors as a behavioral response of fish and invertebrates. A video-based computerized system was used to capture and analyze the behavioral data in real time. Test fish species used were: Chinook salmon, Oncorhynchus tshawytscha; striped bass, Morone saxatilis; steelhead, Salmo gairdneri; fathead minnow, Pimephales promelas; and the invertebrate Daphnia magna. Both attraction and avoidance responses were observed at different concentrations of the toxicant and effluents studied with responses detected as low as 500:1 for effluents. Daphnia was especially sensitive to phenol (0.5 mg/L), while salmonids responded to 10−4 olfactory stimulant (amino acid mix). A discussion of behavioral testing as a tool to assess potential impacts of toxicants, mixed effluents, and olfactory masking is presented.


    aquatic toxicology, avoidance/preference, behavioral bioassay, olfactory stimulants, phenol

    Author Information:

    Smith, EH
    Sebastopol, CA

    Bailey, HC
    Research associate, University of California, Davis, CA

    Committee/Subcommittee: E47.10

    DOI: 10.1520/STP16764S