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    STP1216

    Correlation with Fetax of a Cellular Bioassay -Cell Health Assay of Water Quality -CHAWQ

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    Abstract

    We used albino Xenopus laevis embryos to test the correlation of a short-term, cellular, multiple-endpoint assay (Cell Health Assay of Water Quality- CHAWQ) with a 96 hour assay FETAX (Frog Embryo Teratogenicity Assay: Xenopus). We describe the hardware and instrumentation to perform the assay. We also describe the endpoints and methods to measure them. Exemplary endpoint measurements are presented: 6-Aminonicotinamide produced a decrease in the membrane potential of the embryo as measured by dye fluorescence. A potato glycoalkaloid, α-chaconine, increased dye fluorescence due to intracellular pH. Measurements of DNA using fluorescent dyes showed a normal increase in DNA until gastrulation. A clastogen — triethylenemelamine- was detected with hex-bisbenzamide dye. These data suggest that the CHAWQ assay may contain endpoints that predict acute or chronic macroscopic effects on an organism.

    Keywords:

    FETAX, acute bioassay, Di-4-ANEPPS, Xenopus laevis, chaconine, 6-aminonicotinamide, triethylenemelamine, bisbenzamide, thiazole orange, CHAWQ


    Author Information:

    Blankemeyer, JT
    Associate Professor, Graduate Student, and Associate Dean for Research, Oklahoma State University, Stillwater, OK

    Stringer, BK
    Associate Professor, Graduate Student, and Associate Dean for Research, Oklahoma State University, Stillwater, OK

    Bantle, JA
    Associate Professor, Graduate Student, and Associate Dean for Research, Oklahoma State University, Stillwater, OK

    Friedman, M
    Senior Research Scientist, USDA-ARS, Albany, CA


    Committee/Subcommittee: E47.04

    DOI: 10.1520/STP13151S