SYMPOSIA PAPER Published: 01 January 2004
STP11630S

Development and Validation of a Detection Method for a Broad Range of Human Papillomavirus Types

Source

Human papillomaviruses (HPVs) are viruses that are almost unique in their tropism for human skin cells; they also have the potential to cause severe pathology. These viruses present a significant risk to contaminate tissue engineered skin replacement products that use human-derived skin cells as part of their manufacture. Eight pairs of primers and appropriate DNA oligonucleotide probes were designed from a relatively conserved region of the LI open reading frame of HPV. TaqMan® PCR using these primer/probe sets was found to be capable of detecting HPV-DNA in a background of cellular material likely to be present in skin replacement products. The assay is more sensitive than any currently described in the scientific literature and can be completed in a single working day making it suitable for testing the short shelf life that many of these products have.

Author Information

Galbraith, DN
Q-One Biotech Ltd., West of Scotland Science Park, Glasgow, United Kingdom
Collins, T
Q-One Biotech Ltd., West of Scotland Science Park, Glasgow, United Kingdom
Black, J
Q-One Biotech Ltd., West of Scotland Science Park, Glasgow, United Kingdom
McManus, B
Q-One Biotech Ltd., West of Scotland Science Park, Glasgow, United Kingdom
McMutrie, D
Q-One Biotech Ltd., West of Scotland Science Park, Glasgow, United Kingdom
Lovatt, A
Q-One Biotech Ltd., West of Scotland Science Park, Glasgow, United Kingdom
Price: $25.00
Contact Sales
Related
Reprints and Permissions
Reprints and copyright permissions can be requested through the
Copyright Clearance Center
Details
Developed by Committee: F04
Pages: 12–19
DOI: 10.1520/STP11630S
ISBN-EB: 978-0-8031-5497-1
ISBN-13: 978-0-8031-3471-3