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This article presents the structural testing and analyses of a segmented horizontal axis wind turbine blade. The blade’s mass and center of gravity are first determined. Free vibration tests are performed, and the natural frequencies are obtained. Static bending tests in flapwise and edgewise directions are conducted with acoustics emission monitoring. The segmented blade was able to sustain the test loads. However, some superficial defects appeared and were detected by the acoustics emission sensors. Finally, the resonant fatigue test is performed for ten million cycles. The variation of the stiffness against the loading cycles is used to diagnose the initiation of fatigue cracks. Based on the testing results, some modifications are proposed in the design and manufacturing approaches to avoid the noted defects.