Journal Published Online: 01 November 1984
Volume 12, Issue 6

Evaluation of the Ultrasonic Method for Solubilizing Before Liquid Scintillation Counting

CODEN: JTEVAB

Abstract

Adult Daphnia magna were exposed to 14C-labeled phenol and tissues analyzed for 14C uptake by three methods: (1) tissue solubilizer, (2) tissue solubilizer plus sonication, and (3) sonication alone. Analysis by liquid scintillation counting revealed that measurements of total activity among treatments were not significantly different (α ≤ 0.10) at two count levels. Sonicated samples showed less variation than tissue samples that were solubilized.

Author Information

Dauble, DD
Pacific Northwest Laboratory, Richland, WA
Hanf, RW
Pacific Northwest Laboratory, Richland, WA
Carlile, DW
Pacific Northwest Laboratory, Richland, WA
Pages: 2
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Stock #: JTE10744J
ISSN: 0090-3973
DOI: 10.1520/JTE10744J