Journal Published Online: 06 April 2005
Volume 50, Issue 3

Forensic Casework Analysis Using the HVI/HVII mtDNA Linear Array Assay

CODEN: JFSCAS

Abstract

The mitochondrial hypervariable regions I and II have proven to be a useful target for analysis of forensic materials, in which the amount of DNA is limited or highly degraded. Conventional mitochondrial DNA (mtDNA) sequencing can be time-consuming and expensive, limitations that can be minimized using a faster and less expensive typing assay.We have evaluated the exclusion capacity of the linear array mtDNA HVI/HVII region-sequence typing assay (Roche Applied Science) in 16 forensic cases comprising 90 samples. Using the HVI/HVII mtDNA linear array, 56% of the samples were excluded and thus less than half of the samples require further sequencing due to a match or inconclusive results. Of all the samples that were excluded by sequence analysis, 79% could be excluded using the HVI/HVII linear array alone. Using the HVI/HVII mtDNA linear array assay, we demonstrate the potential to decrease sequencing efforts substantially and thereby reduce the cost and the turn-around time in casework analysis.

Author Information

Divne, A-M
Uppsala University, Department of Genetics and Pathology, Rudbeck Laboratory, Uppsala, Sweden
Nilsson, M
Uppsala University, Department of Genetics and Pathology, Rudbeck Laboratory, Uppsala, Sweden
Calloway, C
Roche Molecular Systems, Alameda, CA
Reynolds, R
Roche Molecular Systems, Alameda, CA
Erlich, H
Roche Molecular Systems, Alameda, CA
Allen, M
Uppsala University, Department of Genetics and Pathology, Rudbeck Laboratory, Uppsala, Sweden
Pages: 7
Price: $25.00
Related
Reprints and Permissions
Reprints and copyright permissions can be requested through the
Copyright Clearance Center
Details
Stock #: JFS2004505
ISSN: 0022-1198
DOI: 10.1520/JFS2004505