Abstract

Genomic DNA was isolated from buccal swabs by the QIAamp DNA Mini Kit (QIAGEN). The primers used for amplification and sequencing of exon 6 and 7 of ABO gene were suggested by Lee and Chang (1), and Ogasawara et al. (2), respectively. About 1-2 ng of genomic DNA was used for PCR in 25 μL reaction volume. PCR mixture contained 10 mM Tris-HCl pH 8.5, 50 mM KCl, 1.5 mM MgCl2, 200 μM of each dNTP, 0.25 μM of each primer, and 1 U of AmpliTaq Gold DNA Polymerase (Applied Biosystems). Thermal cycling was performed initially at 95°C for 10 min, then 35 cycles consisting of 95°C for 1 min, 60°C for 1 min for exon 6 and 63°C for 1 min for exon 7, 72°C for 1 min, followed by 10 min extension at 72°C in a GeneAmp PCR System 9600 (Perkin Elmer).

Author Information

Shin, K
Yonsei University, Seoul, Korea Human Identification Research Institute, Yonsei University, Seoul, Korea
Yang, Y
Yonsei University, Seoul, Korea
Choi, J
Yonsei University, Seoul, Korea Human Identification Research Institute, Yonsei University, Seoul, Korea
Yoon, C
Chosun University, Kwangju, Korea Human Identification Research Institute, Yonsei University, Seoul, Korea
Park, K
Yonsei University, Seoul, Korea Human Identification Research Institute, Yonsei University, Seoul, Korea
Cho, S
Yonsei University, Seoul, Korea Human Identification Research Institute, Yonsei University, Seoul, Korea
Kim, C
Yonsei University, Seoul, Korea Human Identification Research Institute, Yonsei University, Seoul, Korea
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Stock #: JFS2003169
ISSN: 0022-1198
DOI: 10.1520/JFS2003169