Abstract

A total 107 EDTA-blood samples was collected from unrelated males of Han population in Chengdu of China. DNA was extracted utilizing the Chelex-100 method (1). The allelic variation at the two Y-STR loci named as DYS544 and DYS587 were analyzed by PCR amplification. Each PCR reaction contained 2-5 ng human genome, 1 × Taq buffer, 1.5 mM MgCl2, 200 μM each dNTP (Pharmacia Biotech, Sweden), 2 U Taq polymerase (Promega Corporation), 0.3 μM each primer, in a total volume of 37.5 μL. PCR amplifications were carried out in a GeneAmp PCR System 9600 (Perkin-Elmer) with pre-denaturing for 2 min at 94°C, followed by 33 cycles of denaturing for 30 s at 94°C, annealing for 60 s at 58°C, and extension for 30 s at 72°C.

Author Information

Dai, HL
Institute of Forensic Medicine, Sichuan University (West China University of Medical Sciences), Sichuan, P. R. China
Wang, XD
Institute of Forensic Medicine, Sichuan University (West China University of Medical Sciences), Sichuan, P. R. China
Dong, JG
Institute of Forensic Medicine, Sichuan University (West China University of Medical Sciences), Sichuan, P. R. China
Zhang, HJ
Center of Forensic Sciences, Public Security Bureau of Sichuan Province, Sichuan, P. R. China
Hou, YP
Institute of Forensic Medicine, Sichuan University (West China University of Medical Sciences), Sichuan, P. R. China
Li, YB
Institute of Forensic Medicine, Sichuan University (West China University of Medical Sciences), Sichuan, P. R. China
Wu, J
Institute of Forensic Medicine, Sichuan University (West China University of Medical Sciences), Sichuan, P. R. China
Zhang, J
Institute of Forensic Medicine, Sichuan University (West China University of Medical Sciences), Sichuan, P. R. China
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Stock #: JFS2003167
ISSN: 0022-1198
DOI: 10.1520/JFS2003167