Published Online: 2003
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A total of 74 blood samples were collected from unrelated males of Han ethnic group in Chongqing of China. DNA was extracted by utilizing Chelex method (1). Each PCR reaction contained 2-10 ng DNA, 1 X Taq buffer, 1.5 mM MgCl2, 200 RM each dNTP (Pharmacia Biotech, Sweden), 1.5 U Taq polymerase (NEB, UK), 0.3 RM each primer. PCR amplifications were performed in a GeneAmp PCR System 9600 (Perkin-Elmer, Foster City, CA),with denaturing for 2 min at 94°C, followed by 30 cycles of 94°C for 50 s, 58°C for 50 s and 72°C for 30 s. PCR products were analyzed by horizontal non-denaturing polyacrylamide gel electrophoresis with discontinuous buffer system, gels were silver stained (2). Alleles were designated according to recommendation of the International Society of Forensic Genetics (3). The gene diversities, the haplotypes diversity, and the standard errors of diversity were calculated in accordance with Hou's method (4).
Stock #: JFS2003096