Abstract

Whole blood samples were collected from 109 unrelated males of Han ethnic group in Sichuan of China. DNA was extracted using Chelex method (1). The volume of PCR reaction for each locus was 25 µL, which contained 1-5 ng human genome, 1 X Taq buffer, 1.5 mM MgCl2, 200 R M each dNTP (Pharmacia Biotech, Sweden), 1 U Taq polymerase (Applied Biosystems, Foster City, CA), 0.25 R M each primer (Each forward primer was 5' FAM labeled). PCR amplifications were carried out in a GeneAmp PCR System 9600 (Perkin-Elmer, Foster City, CA). The cycling protocols were: 94°C for 1 min, followed by 30 cycles of 94°C for 30 s, 59°C for 30 s, 72°C for 40 s, and extension at 72°C for 30 min. Typing were performed using denaturing capillary gel electrophoresis on an ABI PRISM 310 Genetic Analyzer with laser-induced fluorescence detection. Alleles were designated according to recommendation of the DNA commission of the International Society of Forensic

Author Information

Zhang, HJ
Bureau of Public Security of Sichuan Province, Chengdu, Sichuan, P. R. China
Shen, YH
Bureau of Public Security of Sichuan Province, Chengdu, Sichuan, P. R. China
Zhu, QF
Sichuan University (West China University of Medical Sciences), Chengdu, Sichuan, P. R. China
Wang, QH
Bureau of Public Security of Sichuan Province, Chengdu, Sichuan, P. R. China
Ji, Q
Sichuan University (West China University of Medical Sciences), Chengdu, Sichuan, P. R. China
Tang, JP
Sichuan University (West China University of Medical Sciences), Chengdu, Sichuan, P. R. China
Liao, J
Bureau of Public Security of Sichuan Province, Chengdu, Sichuan, P. R. China
Lin, YG
Bureau of Public Security of Sichuan Province, Chengdu, Sichuan, P. R. China
Hon, YP
Sichuan University (West China University of Medical Sciences), Chengdu, Sichuan, P. R. China
Pages: 2
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Stock #: JFS2003082
ISSN: 0022-1198
DOI: 10.1520/JFS2003082