Published Online: 2003
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The allele frequencies for the loci D2S1338, D19S433, Penta D, and Penta E were determined in a Spanish population. Whole blood samples were obtained from 187-208 unrelated donors from Central Spain. Genomic DNA was extracted by a standard phenol/chloroform extraction procedure. PCR amplification was performed using the AmpFℓSTR SGM Plus D2S1338 and D19S433 loci-amplification kit (Applied Biosystems, Foster City, CA) and PowerPlex 16 Penta D and Penta E—amplification kit (Promega Corporation, Madison, WI) following manufacturer's instructions. The amplified products were separated and detected using the ABI 377 DNA sequencer (Applied Biosystems, Foster City, CA). Alleles were classified according to the recommenda- tions of the ISFG (1). Statistical analysis was performed as previ- ously reported (2). The complete dataset is available to any interested researcher upon request from the corresponding author.
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