Journal Published Online: 01 March 2003
Volume 48, Issue 2

Distributions of Allelic Frequencies and Haplotypes of Two Novel Y-Chromosome STR in a Chinese Population

CODEN: JFSCAS

Abstract

Whole blood obtained by venipuncture from 107 unrelated males of the Han ethnic group in Chengdu, China. DNA was extracted using the Chelex method (1). The reaction volume of PCR was 37.5 μL, containing 2–4 ng human genome DNA, 200 μM each dNTP (Pharmacia, Sweden), 1.5μ Taq polymerase (Promega, Madison, WI), 3.75 μL 10 × buffer, Mg2+ 1.5 mM, 1.6 μg/mL BSA, 0.3 μM each primer. Amplification reactions were carried out in a Perkin Elmer 9600 (Foster City, CA) with pre-denaturing for 2 min at 94°C, followed by 35 cycles of denaturing for 40 s at 94°C, annealing for 40 s at 58°C and extension for 25 s at 72°C. The PCR products were analyzed by horizontal non-denaturing polyacrylamide gel electrophoresis with discontinuous buffer system and visualized by silver staining (2). Data of population genetics and forensic science were analyzed according to Hou's method (3).

Author Information

Zhu, QF
Sichuan University, Sichuan, P. R. China
Tang, JP
Sichuan University, Sichuan, P. R. China
Gao, YZ
Sichuan University, Sichuan, P. R. China
Ying, BW
Sichuan University, Sichuan, P. R. China
Hou, YP
Sichuan University, Sichuan, P. R. China
Li, YB
Sichuan University, Sichuan, P. R. China
Wu, J
Sichuan University, Sichuan, P. R. China
Ji, Q
Sichuan University, Sichuan, P. R. China
Pages: 1
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Stock #: JFS2002381
ISSN: 0022-1198
DOI: 10.1520/JFS2002381