Journal Published Online: 01 September 2003
Volume 48, Issue 5

A Novel Multiplex PCR System Consisting of Y-STRs DYS441, DYS442, DYS443, DYS444, and DYS445

CODEN: JFSCAS

Abstract

We have developed a new sensitive multiplex PCR system consisting of five male-specific and polymorphic tetranucleotide STRs-DYS441 (GDB: 10013873), DYS442 (GDB: 10030304), DYS443 (GDB: 10807127), DYS444 (GDB: 10807128), and DYS445 (GDB: 10807129) on the Y chromosome. Fifty pg DNA per 10 µL reaction volume was required for the correct typing of five STRs. Using this system, the five Y-STRs were correctly typed from blood and semen stains that had been stored for several years at room temperature.

Author Information

Iida, R
Fukui Medical University, Fukui, Japan
Sawazaki, K
Fukui Medical University, Fukui, Japan Scientific Investigation Laboratory, Fukui, Japan
Ikeda, H
Fukui Medical University, Fukui, Japan
Miyamoto, T
Fukui Medical University, Fukui, Japan
Tsubota, E
Fukui Medical University, Fukui, Japan
Takatsuka, H
Fukui Medical University, Fukui, Japan
Masuyama, M
Fukui Medical University, Fukui, Japan
Matsuki, T
Fukui Medical University, Fukui, Japan
Yasuda, T
Fukui Medical University, Fukui, Japan
Kishi, K
Gunma University Graduate School of Medicine, Maebashi, Gunma, Japan
Pages: 3
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Stock #: JFS2002379
ISSN: 0022-1198
DOI: 10.1520/JFS2002379