Published Online: 2003
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The samples for database studies were obtained from unrelated males from the indicated population groups residing in the state of Connecticut and were anonymized before analysis. The DNA from blood samples was extracted using the QIAamp® DNA blood MiniKit (Qiagen, Valencia, CA) following the recommended procedures. The quantity of human DNA was determined by slot blot hybridization using the QuantiBlot kit (Applied Biosystems, Foster City, CA) and following the manufacturer's recommended protocols. The Y-PLEX™6 system (ReliaGene Technologies, Inc., New Orleans, LA) was used for the amplification of the Y short tandem repeat (STR) loci. PCR amplification and the analysis of amplified product were performed as recommended by the manufacturer and as described in Sinha et al. (1).
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