Abstract

DNA was isolated from blood samples obtained from 150 healthy male individuals by standard phenol/chloroform procedure (3). Custom-made primers were labeled with 5'-FAM and JOE fluorescent dyes. PCR amplification for all the eight Y-STRs namely, DYS19, DYS385, DYS389I, DYS389II, DYS390, DYS391, DYS392, and DYS393, was carried out with a 25-ng template DNA in two multiplex reactions, standardized in the laboratory, using primers as described elsewhere (4,5). Amplified PCR products were run on a 5% polyacrylamide gel using a ABI 377 Automated DNA Sequencer. Sizing of the products was done using the ABI 377 Genescan Analysis Software version (3.1).

Author Information

Sahoo, S
Central Forensic Science Laboratory, Kolkata
Chainy, GBN
Utkal University, Bhubaneswar
Kashyap, VK
Central Forensic Science Laboratory, Kolkata
Pages: 2
Price: $25.00
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Stock #: JFS20022310
ISSN: 0022-1198
DOI: 10.1520/JFS20022310