Journal Published Online: 01 January 2003
Volume 48, Issue 1

DNA Profiling of Azoospermic Semen Samples from Vasectomized Males by Using Y-PLEX™6 Amplification Kit

CODEN: JFSCAS

Abstract

Post-vasectomized azoospermic semen samples (N = 6) were analyzed for short tandem repeats (STR) on the Y-chromosome by using Y-PLEX™6 and the 310 Genetic Analyzer. We have observed a wide variation in the yield of extracted DNA from 12.5-1000 ng. This variation was attributed to the number of epithelial and/or white blood cells that are present in these azoospermic samples. DNA profiles of these vasectomized males were obtained for all six Y-STR loci, namely DYS393, DYS19, DYS389II, DYS390, DYS391, and DYS385 amplified by using the Y-PLEX™6.

Author Information

Shewale, JG
ReliaGene Technologies, Inc., New Orleans, LA
Sikka, SC
Tulane University, New Orleans, LA
Schneida, E
ReliaGene Technologies, Inc., New Orleans, LA
Sinha, SD
ReliaGene Technologies, Inc., New Orleans, LA Tulane University, New Orleans, LA
Pages: 3
Price: $25.00
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Stock #: JFS2002127
ISSN: 0022-1198
DOI: 10.1520/JFS2002127