Journal Published Online: 01 November 2002
Volume 47, Issue 6

Enantiomeric Separation of Methamphetamine and Related Analogs by Capillary Zone Electrophoresis: Intelligence Study in Routine Methamphetamine Seizures

CODEN: JFSCAS

Abstract

A method for simultaneous enantiomeric separation of ephedrine, pseudoephedrine, and methamphetamine (MA) in a single run by simple capillary zone electrophoresis (CZE) with β-cyclodextrin as a chiral selector is described. The effects of the buffer pH, phosphate concentration, β-cyclodextrin concentration, voltage and temperature on the peak resolution were examined. Good enantiomeric resolution was attained for each analyte under our optimized conditions: 15 mM β-cyclodextrin, 300 mM NaH2PO4 at pH 2.5 with an uncoated capillary (64.5 cm × 50 m), applied potential at 20 kV and temperature at 30°C. Ultraviolet (UV) detection at a fixed wavelength (200 nm) was employed using a diode array detector. Using phentermine as an internal standard, migration times for all analytes are reproducible within 0.1% for intra-day and 0.6% for inter-day runs. Application of this method to the analysis of confiscated drugs is discussed.

Author Information

Cheng, WC
Homantin Government Offices, Homantin, People's Republic of China
Lee, WM
Homantin Government Offices, Homantin, People's Republic of China
Chan, MF
Homantin Government Offices, Homantin, People's Republic of China
Tsui, P
Homantin Government Offices, Homantin, People's Republic of China
Dao, KL
Homantin Government Offices, Homantin, People's Republic of China
Pages: 5
Price: $25.00
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Stock #: JFS15556J
ISSN: 0022-1198
DOI: 10.1520/JFS15556J