Journal Published Online: 01 May 2001
Volume 46, Issue 3

Validation of the AmpFℓSTR™ Profiler Plus PCR Amplification Kit for Use in Forensic Casework

CODEN: JFSCAS

Abstract

According to TWGDAM guideline 4.5 (1), prior to implementing a new DNA analysis procedure or an existing DNA analysis procedure developed by another laboratory, the forensic laboratory must first demonstrate reliability of the procedure inhouse. Seven phases were designed to validate the use of the AmpFℓSTR Profiler Plus PCR Amplification Kit, as well as the PE Applied Biosystems 310 Genetic Analyzer. This report summarizes the results obtained for each of the seven phases of the validation study which included the following evaluations: polymer, reproducibility, sensitivity, stutter, precision, mixtures and nonprobative casework.

Author Information

Frank, WE
Illinois State Police, Research and Development Laboratory, Springfield, IL
Llewellyn, BE
Illinois State Police, Research and Development Laboratory, Springfield, IL
Fish, PA
Illinois State Police, Research and Development Laboratory, Springfield, IL
Riech, AK
Illinois State Police, Research and Development Laboratory, Springfield, IL
Marcacci, TL
Illinois State Police, Research and Development Laboratory, Springfield, IL
Gandor, DW
Illinois State Police, Research and Development Laboratory, Springfield, IL
Parker, D
Illinois State Police, Research and Development Laboratory, Springfield, IL
Carter, RR
Illinois State Police, Research and Development Laboratory, Springfield, IL
Thibault, SM
Illinois State Police, Research and Development Laboratory, Springfield, IL
Pages: 5
Price: $25.00
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Stock #: JFS15017J
ISSN: 0022-1198
DOI: 10.1520/JFS15017J