Journal Published Online: 01 July 2000
Volume 45, Issue 4

Sequence Variation of New Alleles at the Short Tandem Repeat D19S253 Locus

CODEN: JFSCAS

Abstract

This paper reports the sequences of two new alleles identified in a population database study on the short tandem repeat D19S253 locus. A Portuguese Caucasian population and a Portuguese African population were studied. Forty-four selected alleles were sequenced and 11 different alleles were found. All the sequenced alleles shown to possess a simple tetranucleotide GATA repeat region structure. The two new alleles, alleles 6 and 16, follow the simple repeat pattern. During paternity investigation casework, 1028 meiosis were analyzed and five isolated genetic incompatibilities detected. In one case, a non-detectable allele with the used set of primers could be the explanation. In the other four cases, single-step mutations could be considered. The mutation rate obtained for this locus was 3.89 × 10−3.

Author Information

Brito, RM
Institute of Legal Medicine, Lisbon, Portugal
Ribeiro, T
Institute of Legal Medicine, Lisbon, Portugal
Viriato, L
Institute of Legal Medicine, Lisbon, Portugal
Vieira-Silva, C
Institute of Legal Medicine, Lisbon, Portugal
Espinheira, R
Institute of Legal Medicine, Lisbon, Portugal
Pinto-Ribeiro, I
Institute of Legal Medicine, Lisbon, Portugal Faculty of Medicine, University of Lisbon, Portugal
Geada, H
Institute of Legal Medicine, Lisbon, Portugal Faculty of Medicine, University of Lisbon, Portugal
Pages: 3
Price: $25.00
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Stock #: JFS14803J
ISSN: 0022-1198
DOI: 10.1520/JFS14803J