Journal Published Online: 01 September 1997
Volume 42, Issue 5

HumCD4—Validation of a STR System for Forensic Purposes in an Austrian Caucasian Population Sample

CODEN: JFSCAS

Abstract

The short tandem repeat system HumCD4 was amplified by the polymerase chain reaction (PCR) on blood samples from 304 unrelated Austrian Caucasians and analyzed by horizontal, non-denaturing polyacrylamide electrophoresis. The mean exclusion chance was 0.417, the discriminating power 0.850 and the heterozygosity rate 0.628. The observed phenotype distribution is in Hardy-Weinberg equilibrium. In 100 families (200 meioses) no mutations were found. Sufficient amplification could be achieved with as little as 80 pg of high molecular weight cell-line DNA. In a degradation experiment DNA extracted from bloodstains stored for up to 28 days in a moist chamber and DNA boiled for up to 18 min could be amplified. A duplex PCR with TH01 is proposed.

Author Information

Klintschar, M
Institute for Legal Medicine, University, Graz, Austria
Crevenna, R
Institute for Legal Medicine, University, Graz, Austria
Pages: 4
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Stock #: JFS14228J
ISSN: 0022-1198
DOI: 10.1520/JFS14228J