Journal Published Online: 01 January 1996
Volume 41, Issue 1

Using Multiplex PCR Amplification and Typing Kits for the Analysis of DNA Evidence in a Serial Killer Case

CODEN: JFSCAS

Abstract

Analysis of DNA evidence in a serial killer case was performed using the AmpliType® HLA-DQ alpha-, AmpliType® PM-, and the GenePrint™ STR Multiplex System PCR Amplification Kits. In addition, a sex typing procedure using the X-Y homologous gene amelogenin was carried out. DNA profiles from a single hair with attached sheath material, recovered from underneath the seat cover of the suspect's car seat were compared with DNA profiles derived from reference head hairs from a homicide victim. From the evidentiary sample only 9 ng of human DNA could be recovered. In a sample, where the quantity of DNA becomes a critical issue a powerful route is the simultaneous amplification of several loci (multiplex PCR). This is the first report where commercially available multiplex PCR amplification and typing kits have been introduced for the analysis of DNA evidence in a serial killer case and the analysis has been admitted in court.

Author Information

Hochmeister, MN
DNA Identity Laboratory, Institute of Legal Medicine, University of Berne, Switzerland
Budowle, B
Forensic Science Research and Training Center, Laboratory Division, Federal Bureau of Investigation Academy, Quantico, VA
Eisenberg, A
University of North Texas Health Science Center at Fort Worth, Fort Worth, TX
Borer, UV
DNA Identity Laboratory, Institute of Legal Medicine, University of Berne, Switzerland
Dirnhofer, R
Institute of Legal Medicine, University of Berne, Switzerland
Pages: 8
Price: $25.00
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Stock #: JFS13916J
ISSN: 0022-1198
DOI: 10.1520/JFS13916J