Journal Published Online: 01 July 1995
Volume 40, Issue 4

Confirmation of the Identity of Human Skeletal Remains Using Multiplex PCR Amplification and Typing Kits

CODEN: JFSCAS

Abstract

The identity of human skeletal remains found in a wooded area approximately one year after the person was reported missing was provisionally established by routine methods and circumstantial evidence. Multiplex PCR systems—the AmpliType® PM PCR Amplification and Typing Kit and the GenePrint™ STR Triplex Amplification and Typing Kit—were used to confirm the identification. DNA profiles from femur bone from the remains were compared with profiles derived from head hairs from a hairbrush recovered in the missing woman's apartment. In addition, a sex typing procedure using the X-Y homologous gene amelogenin was carried out. This is the first report of a case using commercially available multiplex PCR amplification and typing kits to confirm the identity of skeletal remains.

Author Information

Hochmeister, MN
DNA Identity Laboratory, Institute of Legal Medicine, University of Berne, Berne, Switzerland
Budowle, B
Forensic Science Research and Training Center, Laboratory Division, Federal Bureau of Investigation Academy, Quantico, VA
Borer, UV
DNA Identity Laboratory, Institute of Legal Medicine, University of Berne, Berne, Switzerland
Rudin, O
DNA Identity Laboratory, Institute of Legal Medicine, University of Berne, Berne, Switzerland
Bohnert, M
Institute of Legal Medicine, University of Freiburg im Breisgau, Germany
Dirnhofer, R
DNA Identity Laboratory, Institute of Legal Medicine, University of Berne, Berne, Switzerland
Pages: 5
Price: $25.00
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Stock #: JFS13855J
ISSN: 0022-1198
DOI: 10.1520/JFS13855J