Journal Published Online: 01 May 1995
Volume 40, Issue 3

Sensitive Detection of Strychnine in Biological Samples by Gas Chromatography with Surface Ionization Detection

CODEN: JFSCAS

Abstract

Strychnine was found measurable with high sensitivity by gas chromatography (GC) with surface ionization detection (SID) and using brucine as the internal standard. The calibration curve was linear in the range of 62.5 to 1000 pg, on column. The detection limit of strychnine was about 50 pg on column (2.5 ng/mL sample); the sensitivity was 70 times higher than that reported by high-performance liquid chromatography. A detailed procedure for extraction of strychnine from human whole blood and urine was established using Sep-Pak C18 cartridges; the recovery of strychnine and brucine, which had been spiked in both body fluids, was about 90%.

Author Information

Ishii, A
Hamamatsu University School of Medicine, Hamamatsu, Japan
Hattori, H
Aichi Medical University, Aichi, Japan
Seno, H
Hamamatsu University School of Medicine, Hamamatsu, Japan
Kumazawa, T
Showa University School of Medicine, Tokyo, Japan
Suzuki, O
Hamamatsu University School of Medicine, Hamamatsu, Japan
Pages: 3
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Stock #: JFS13810J
ISSN: 0022-1198
DOI: 10.1520/JFS13810J