Journal Published Online: 01 September 1994
Volume 39, Issue 5

Amplification of cDNA via RT-PCR Using RNA Extracted from Postmortem Tissues

CODEN: JFSCAS

Abstract

Analysis of cDNA derived from messenger RNA is of advantage over using genomic DNA in genetic analysis of large genes, especially those with lengthy intron sequences. However, because of its instability and rapid degradation, RNA extraction from postmortem tissues has not been attempted. Here, we report the successful extraction of intact mRNA from various postmortem tissues from accidental and sudden death cases. Subsequently with reverse transcriptase-polymerase chain reaction (RT-PCR), we were able to amplify cDNA fragments of different lengths up to 0.9 kb. The described method therefore provides a useful tool in genetic analysis of postmortem tissues.

Author Information

Phang, T-W
Department of Community, Occupational and Family Medicine, Singapore
Shi, C-Y
Department of Community, Occupational and Family Medicine, Singapore
Chia, J-N
Institute of Molecular and Cell Biology; National University of Singapore
Ong, C-N
Department of Community, Occupational and Family Medicine, Singapore
Pages: 5
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Stock #: JFS13713J
ISSN: 0022-1198
DOI: 10.1520/JFS13713J