(Received 22 April 1992; accepted 17 May 1993)
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This paternity study was performed with trios in which the putative father was not the biological father (NBF), in order to evaluate the effectiveness of HLA-A and -B phenotyping for routine testing.
All 372 generated trios had ABO, Rh, MNS, Kell, Duffy, Kidd and HLA (A and B) systems tested.
HLA-A and -B phenotyping directly excluded 81.73% of NBF. Red blood cell markers excluded 8.82% of NBF missed by HLA; only 0.81% of NBF were not identified by the markers used.
Each laboratory engaged in disputed paternity testing should evaluate its actual performance in detecting non-biological fathers and its exclusion rates in real trios. These data together can be used as a guide of mother's reliability.
University of São Paulo, São Paulo,
Stock #: JFS13554J