Journal Published Online: 01 May 1993
Volume 38, Issue 3

Purification of Forensic Specimens for the Polymerase Chain Reaction (PCR) Analysis

CODEN: JFSCAS

Abstract

Purification methods of deoxyribonucleic acid (DNA) from degraded and contaminated forensic samples were investigated for polymerase chain reaction (PCR) analysis. DNA extracted from putrefied tissue or bloodstains sometimes contained the copurified contaminant, that was identified as the porphyrin compound (hematin). When contaminated but less degraded DNA was analyzed by PCR, it was necessary to eliminate the impurity by anion exchange column chromatography or chelating resin preparation, and ultrafiltration using Centricon microconcentrators. When highly degraded DNA was analyzed, trace amounts of high molecular weight DNA was recovered by electroelution method, and then further purified by both column chromatography and ultrafiltration. From thus purified samples, the amelogenin gene for sex determination could be amplified by dual PCR technique.

Author Information

Akane, A
Shimane Medical University, Izumo, Japan
Shiono, H
Shimane Medical University, Izumo, Japan
Matsubara, K
Shimane Medical University, Izumo, Japan
Nakamura, H
Criminal Investigation Laboratory, Shimane Prefectural Police Headquarters, Matsue, Japan
Hasegawa, M
Criminal Investigation Laboratory, Shimane Prefectural Police Headquarters, Matsue, Japan
Kagawa, M
Criminal Investigation Laboratory, Shimane Prefectural Police Headquarters, Matsue, Japan
Pages: 11
Price: $25.00
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Stock #: JFS13457J
ISSN: 0022-1198
DOI: 10.1520/JFS13457J