Journal Published Online: 01 November 1992
Volume 37, Issue 6

The Application of Immunoblotting to the Phenotyping of Haptoglobin

CODEN: JFSCAS

Abstract

An immunoblotting method for phenotyping haptoglobin in serum and blood-stains has been developed. Haptoglobin isoproteins were separated by polyacrylamide gradient gel electrophoresis and then transferred to nitrocellulose by electroblotting. The use of 1 mm gels facilitated more rapid and effective transfer than conventional 3 mm thick gels. Nitrocellulose blots were developed by double antibody enzyme immunoassay. The detection limit for serum and bloodstains was improved 16 times compared to conventional staining using O-tolidine. The method could detect haptoglobin phenotypes from 0.001 μl of whole blood. This detection limit is approximately 8 times lower than that of group specific-component analysis by immunoblotting.

Author Information

Cox, KJ
Forensic Biology section, State Health Laboratory, Brisbane, Queensland, Australia
Thomas, AS
Forensic Biology section, State Health Laboratory, Brisbane, Queensland, Australia
Pages: 4
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Stock #: JFS13355J
ISSN: 0022-1198
DOI: 10.1520/JFS13355J